| Registro completo |
| Provedor de dados: |
Ciência Rural
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| País: |
Brazil
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| Título: |
Brazilian ground beef authentication by multiplex polymerase chain reaction
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| Autores: |
Oliveira,Andrey Carlos do Sacramento de
Pedroso,Silvia Cristina da Silva
Cardilli,Diogo José
Leite,Fábio Pereira Leivas
Ferreira,Gabrielle Virgínia Lopes
Silva,Andréia Silva da
Roos,Talita Bandeira
Moraes,Carina Martins de
Sousa,Roberta Sales
Monteiro,Roseli do Socorro Dias
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| Data: |
2018-01-01
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| Ano: |
2018
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| Palavras-chave: |
Species identification
Bos taurus
Bubalusbubalis
DNA
Adulteration
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| Resumo: |
ABSTRACT: The aim of the present study was to assess the efficacy ofmultiplex PCR in detecting the adulterationof commercially available ground beefvia addition and/orsubstitution ofground buffalo meat. Experimentally adulterated ground beefsamples were prepared in triplicate, and dilutions of DNA from Bos taurus and Bubalusbubalis were prepared to determine the detection limit of the method. Concurrently, 91 ground meatsamples sold as “ground beef” were collected from differentstores in northern Brazil andanalyzed bymultiplex PCR. Buffalo DNA was detected in 17.5% of the collected ground meat samples.Our results showed that multiplex PCR is an efficient method for detectingthe incorporation of groundbuffalo meatatpercentages ranging from 10 to 100% and the incorporation of beef at percentages ranging from0.1 to 100% intoground meat samples.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200652
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| Editor: |
Universidade Federal de Santa Maria
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| Relação: |
10.1590/0103-8478cr20160574
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| Formato: |
text/html
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| Fonte: |
Ciência Rural v.48 n.2 2018
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| Direitos: |
info:eu-repo/semantics/openAccess
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